Detection of subcellular nitric oxide in mitochondria using a pyrylium probe: assays in cell cultures and peripheral blood
Ignacio Muñoz Resta, Begoña Bedrina, Elena Martínez-Planes, Alfredo Minguela, Francisco Galindo
Abstract
SNAP donor and LPS stimulation, respectively). In the second area, mtNOpy has been able to discriminate between NO-generating phagocytes (neutrophils and monocytes) from other leukocytes (NK, B and T cells).
Topics & Concepts
Flow cytometryNitric oxideFluorescenceIntracellularConfocalMitochondrionOrganelleFluorescence microscopeCell cultureConfocal microscopyCell biologyChemistryContext (archaeology)BiophysicsBiologyMolecular biologyBiochemistryGeometryOrganic chemistryGeneticsMathematicsPaleontologyPhysicsQuantum mechanicsMolecular Sensors and Ion DetectionLuminescence and Fluorescent MaterialsSulfur Compounds in Biology