MUC3A promotes non-small cell lung cancer progression via activating the NFκB pathway and attenuates radiosensitivity
Yingming Sun, Xiaoge Sun, Chengcheng You, Shijing Ma, Yuan Luo, Shan Peng, Fang Tang, Xiaoli Tian, Feng Wang, Zhengrong Huang, Hongnv Yu, Yu Xiao, Xiaoyong Wang, Junhong Zhang, Yan Gong, Conghua Xie
Abstract
Mucin 3A (MUC3A) is highly expressed in non-small cell lung cancer (NSCLC), but its functions and effects on clinical outcomes are not well understood. Tissue microarray of 92 NSCLC samples indicated that high levels of MUC3A were associated with poor prognosis, advanced staging, and low differentiation. MUC3A knockdown significantly suppressed NSCLC cell proliferation and induced G1/S accumulation via downregulating cell cycle checkpoints. MUC3A knockdown also inhibited tumor growth in vivo and had synergistic effects with radiation. MUC3A knockdown increased radiation-induced DNA double strain breaks and -H2AX phosphorylation in NSCLC cells. MUC3A downregulation inhibited the BRCA-1/RAD51 pathway and nucleus translocation of P53 and XCRR6, suggesting that MUC3A promoted DNA damage repair and attenuated radiation sensitivity. MUC3A knockdown also resulted in less nucleus translocation of RELA and P53 in vivo. Immunoprecipitation revealed that MUC3A interacted with RELA and activated the NFB pathway via promoting RELA phosphorylation and interfering the binding of RELA to IB. Our studies indicated that MUC3A was a potential oncogene and associated with unfavorable clinical outcomes. NSCLC patients with a high MUC3A level, who should be more frequent follow-up and might benefit less from radiotherapy.