Rational Regulation of Reaction Specificity of Nitrilase for Efficient Biosynthesis of 2-Chloronicotinic Acid through a Single Site Mutation
Andi Dai, Xiaoling Tang, Zhe‐Ming Wu, Jiang-Tao Tang, Ren‐Chao Zheng, Yu‐Guo Zheng
Abstract
NIT was precisely tuned through a single site mutation. The mutant exhibited remarkably enhanced hydrolysis activity without the formation of byproducts, providing a robust biocatalyst for 2-CA biosynthesis with industrial potential.
Topics & Concepts
NitrilaseChemistryBiocatalysisHydrolysisSteric effectsCatalysisStereochemistryActive siteCombinatorial chemistryReaction mechanismOrganic chemistryEnzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationPeptidase Inhibition and Analysis