Base-modified aptamers obtained by cell-internalization SELEX facilitate cellular uptake of an antisense oligonucleotide
Keisuke Tanaka, Takumi Okuda, Yuuya Kasahara, Satoshi Obika
Abstract
RNA levels. In contrast, the activity of the aptamer-ASO conjugate was potentiated when endosomal/lysosomal escape was enhanced by the addition of chloroquine. Thus, we showed that the hydrophobic modification of the nucleobase moiety is useful for developing highly internalizing aptamers and that endosomal/lysosomal escape is important for the intracellular delivery of ASOs by aptamers.
Topics & Concepts
InternalizationAptamerOligonucleotideSystematic evolution of ligands by exponential enrichmentBase (topology)ChemistryCellBiologyCell biologyMolecular biologyDNAGeneticsRNAMathematicsGeneMathematical analysisAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry