Two-photon excited red-green “discoloration” bioprobes for monitoring lipid droplets and lipid droplet-lysosomal autophagy
Mingxuan Liu, Li Xu, Pengfei Zhu, Xin Li, Shan Miao, Wei Jin, Jing Chen, Yong Ling, Xiaoling Zhang
Abstract
the green fluorescence emission of its acid-hydrolyzed product (colocalization constant of 0.90) to track the autophagy dynamic process. In addition, DPABP-BI enabled the specific recognition of fatty substances in zebrafish larvae. In this study, a two-photon excited red light small molecule probe was constructed to identify LDs and track their autophagy dynamic process by changing the fluorescence emission wavelength.
Topics & Concepts
ColocalizationFluorescenceLipid dropletAutophagyBiophysicsStokes shiftTwo-photon excitation microscopyBODIPYExcited stateMaterials scienceChemistryNanotechnologyBiochemistryBiologyCell biologyOpticsPhysicsNuclear physicsApoptosisLipid metabolism and biosynthesisPhotosynthetic Processes and MechanismsMetabolomics and Mass Spectrometry Studies