Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis
Ana Paula Andreolla, Alessandra Abel Borges, Seigo Nagashima, Caroline Busatta Vaz de Paula, Lúcia de Noronha, Nilson Ivo Tonin Zanchin, Juliano Bordignon, Claudia Nunes Duarte dos Santos
Abstract
Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing sporadic outbreaks. Due to the similarity of initial clinical symptoms caused by OROV with other arboviruses found in overlapping geographical areas, differential diagnosis is challenging. As for most neglected tropical diseases, there is a shortage of reagents for diagnosing and studying OROV pathogenesis. We therefore developed and characterized mouse monoclonal antibodies and, one of them recognizes the OROV nucleocapsid in indirect immunofluorescent (IFA) and immunohistochemistry (IHC) assays. Considering that it is the first monoclonal antibody produced for detecting OROV infections, we believe that it will be useful not only for diagnostic purposes but also for performing serological surveys and epidemiological surveillance on the dispersion and prevalence of OROV in Brazil and South America.