CircularRNA circ_0071269 knockdown protects against from diabetic cardiomyopathy injury by microRNA-145/gasdermin A axis
Lanfang Fu, Juyun Zhang, Lin Zhu, Yi Li, Guijun Qin
Abstract
. Moreover, circ_0071269 sponges miR-145 to upregulate GSDMA. A miR-145 inhibitor antagonized the effects of circ_0071269 knockdown on the cellular functions of H9c2 cells, while the effects of miR-145 were abrogated by the overexpression of GSDMA. Meanwhile, knockdown of circ_0071269 attenuated cardiac dysfunction of DM mice. Hence, circ_0071269 may promote the development of DCM through the miR-145/GSDMA axis and thus provide a novel marker for the treatment of DCM.
Topics & Concepts
Gene knockdownViability assayFlow cytometryDownregulation and upregulationMolecular biologyIn vivoDiabetic cardiomyopathyLactate dehydrogenaseChemistryBlotPyroptosismicroRNACellApoptosisBiologyProgrammed cell deathMedicineCardiomyopathyEnzymeInternal medicineBiochemistryHeart failureGeneBiotechnologyCircular RNAs in diseasesInflammasome and immune disordersCardiovascular Disease and Adiposity