Method Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Curcumin in Simulated Nasal Fluid
Sherry Sharma, Jai Bharti Sharma, Shailendra Bhatt, Manish Kumar
Abstract
BACKGROUND: Curcumin is a polyphenolic compound with numerous therapeutic activities. There is no validated method available for the quantitative estimation of curcumin in simulated nasal fluid. OBJECTIVE: The aim of present investigation was to develop a simple and precise UV visible spectrophotometric method for estimation of pure form of curcumin in simulated nasal fluid. METHOD: Suitable solvent system was selected by estimation of curcumin at UV maxima of 421nm in simulated nasal fluid with two surfactants (tween 80 and sodium lauryl sulphate). The double beam UV visible spectrophotometer was used for measurement of absorption. The selected solvent system was further validated according to guidelines of international conference on harmonization (ICH), the analytical parameter like linearity, precision and accuracy etc. were studied. RESULTS: Simulated nasal fluid with tween 80 at 1% concentration satisfied all the conditions relative to Peak quality at the stated wavelength. In developed method, curcumin was found to be linear over selected concentration range of 5 to 60µg/ml with a correlation coefficient of 0.998. The accuracy was found to be in range of 99.51 -100.223%.The precision was found to be less than 2 in terms of % RSD. The LOD & LOQ were 0.3657 & 1.109 respectively. CONCLUSION: The proposed method was found to be simple, sensitive and precise. The most important this method can be used for routine quality control analysis of curcumin with accuracy.