Methylated guanosine and uridine modifications in <i>S. cerevisiae</i> mRNAs modulate translation elongation
Joshua D. Jones, Monika K. Franco, T.J. Smith, Laura R. Snyder, Anna G. Anders, Brandon T. Ruotolo, Robert T. Kennedy, Kristin S. Koutmou
Abstract
Four new mRNA modifications were detected in S. cerevisiae by integrating an improved LC-MS/MS approach with an enhanced mRNA purification and validation process. Codons containing these modifications were further identified to impede translation.
Topics & Concepts
Translation (biology)GuanosineUridineMessenger RNAElongationChemistryRNACell biologyBiochemistryBiologyGeneMaterials scienceMetallurgyUltimate tensile strengthRNA modifications and cancerRNA and protein synthesis mechanismsCancer-related molecular mechanisms research