Characterization of conformational states of the homodimeric enzyme fluoroacetate dehalogenase by <sup>19</sup>F–<sup>13</sup>C two-dimensional NMR
Motasem Suleiman, Geordon A. Frere, Ricarda Törner, Lauren Tabunar, Gaurav Vijay Bhole, Keith Taverner, Nobuyuki Tsuchimura, Dmitry Pichugin, Roman J. Lichtenecker, Oleksandr Vozny, Patrick T. Gunning, Haribabu Arthanari, Adnan Sljoka, R. Scott Prosser
Abstract
C) correlation spectra also reveal a multitude of minor resonances in the apo sample. The role of each tryptophan residue in allosteric communication was validated with computational rigidity transmission allostery analysis, which in this case explores the relative interprotomer communication between all possible tryptophan pairs.
Topics & Concepts
FluoroacetateChemistryDehalogenaseEnzymeStereochemistryBiochemistryEnzyme Structure and FunctionProtein Structure and DynamicsBiochemical and Molecular Research