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6-Bromoindirubin-3′-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model

Song Shen, Yilin Zhang, Songmei Zhang, Bing Wang, Lingling Shang, Jinlong Shao, Meng Lin, Yating Cui, Shengjun Sun, Shaohua Ge

2020ACS Biomaterials Science & Engineering34 citationsDOI

Abstract

-glycolic acid) (PLGA) and hyaluronic acid (HA) emerged as promising biomaterials; thus, we developed a novel HA hydrogel embedded with BIO-encapsulated PLGA microspheres and injected the formulation into the gingival sulcus of mice with experimental periodontitis. The release speed of this system was fast in the first week and followed a sustained release phase until week 4. In vivo experiments showed that this PLGA-BIO-HA hydrogel system can inhibit periodontal inflammation, promote bone regeneration, and induce the expression of bone-forming markers alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and osteocalcin (OCN) in a mouse periodontitis model. Therefore, this PLGA-BIO-HA hydrogel system provides a promising therapeutic strategy for periodontal bone regeneration.

Topics & Concepts

Periodontal ligament stem cellsPLGAPeriodontal fiberOsteocalcinPeriodontitisRegeneration (biology)Alkaline phosphataseChemistryStem cellCell biologyRUNX2Tissue engineeringBiomedical engineeringDentistryBiochemistryBiologyMedicineIn vitroEnzymePeriodontal Regeneration and TreatmentsProteoglycans and glycosaminoglycans researchAdvanced Drug Delivery Systems