Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen
Manon Nayrac, Mathieu Dubé, Gérémy Sannier, Alexandre Nicolas, Lorie Marchitto, Olivier Tastet, Alexandra Tauzin, Nathalie Brassard, Raphaël Lima-Barbosa, Guillaume Beaudoin-Bussières, Dani Vézina, Shang Yu Gong, Mehdi Benlarbi, Romain Gasser, Annemarie Laumaea, Jérémie Prévost, Catherine Bourassa, Gabrielle Gendron‐Lepage, Halima Medjahed, Guillaume Goyette, Gloria-Gabrielle Ortega-Delgado, Mélanie Laporte, Julia Niessl, Laurie Gokool, Chantal Morrisseau, Pascale Arlotto, Jonathan Richard, Justin Bélair, Alexandre Prat, Cécile Tremblay, Valérie Martel‐Laferrière, Andrés Finzi, Daniel E. Kaufmann
Abstract
T cell responses further compared with the first dose, unsupervised clustering of single-cell features reveals phenotypic and functional shifts over time and between cohorts. Integrated analysis shows longitudinal immune component-specific associations, with early T helper responses post first dose correlating with B cell responses after the second dose, and memory T helper generated between doses correlating with CD8 T cell responses after boosting. Therefore, boosting elicits a robust cellular recall response after the 16-week interval, indicating functional immune memory.