A one-pot method based on rolling circle amplification and light‐activated CRISPR/Cas12a reaction for simple and highly sensitive detection of Staphylococcus aureus
Qian Wu, Song Jiang, Yong Huang, Lijun Zhang, Zongying Li, Yongbin Hou, Juan Zhang, Yuwei Wang, Chuanlin Zhu, Dandan Zhou, Weixian Chen, Dandan Li, Dandan Li
Topics & Concepts
CRISPRStaphylococcus aureusLoop-mediated isothermal amplificationRolling circle replicationNucleic acidSensitivity (control systems)ChemistryReaction conditionsChromatographyBiological systemBacteriaBiologyDNABiochemistryGeneGeneticsEngineeringCatalysisElectronic engineeringDNA replicationCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical Detection