Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9
Jun‐Liang Tu, Xinyuan Bai, Yong-Liang Xu, Na Li, Jun‐Wei Xu
Abstract
Functional genomic studies in Ganoderma have been hindered by the absence of adequate genome-engineering tools. Although CRISPR/Cas9 works well for gene disruption and deletion in G. lucidum , targeted gene insertion and replacement have remained a serious challenge due to the low efficiency of HR in these species, although such precise genome modifications, including site mutations, site-specific integrations, and allele or promoter replacements, would be incredibly valuable.
Topics & Concepts
CRISPRGanoderma lucidumCas9GeneBiologyNon-homologous end joiningGeneticsComputational biologyChemistryHomologous recombinationFood scienceFungal Biology and ApplicationsBioeconomy and Sustainability DevelopmentMycorrhizal Fungi and Plant Interactions