Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions
Kento Ono, Hiroki Sakai, Shinichi Tokunaga, Tanjina Sharmin, Taku Michael Aida, Kenji Mishima
Abstract
The particles from gas saturated solutions (PGSS) process were performed to encapsulate lactofer-rin, an iron-binding milk glycoprotein, using supercritical carbon dioxide (scCO2). A natural en-teric polymer, shellac, was used as a coating material of lactoferrin carried out by the PGSS pro-cess. Conditions were optimized by applying different temperatures (20–50 °C) and pressures (8–10 MPa) and the particles were evaluated for particle shape and size, lactoferrin encapsulation ef-ficiency, Fourier transform infrared (FTIR) spectroscopy to confirm lactoferrin entrapment and in vitro dissolution studies at different pH values. Particles with an average diameter of 75.5 ± 7 μm were produced with encapsulation efficiency up to 71 ± 2%. Furthermore, particles that showed high stability in low pH (pH 1.2) and a sustained release over time (t2h = 75%) in higher pH (pH 7.4) suggested an effective encapsulation process for the protection of lactoferrin from gastric di-gestion.