Plant-expressed Zika virus envelope protein elicited protective immunity against the Zika virus in immunocompetent mice
Minna Shin, Hyangju Kang, Kyeong Ryeol Shin, Rangyeon Lee, Kiju Kim, Kyungmin Min, Kyou-Nam Cho, Eun‐Ju Sohn, Kwang Sung Kim, Seok-Hyun Kim, Yang Je Cho, Jeongho Park, Tae‐Wook Hahn
Abstract
Zika virus infection causes multiple clinical issues, including Guillain-Barré syndrome and neonatal malformation. Vaccination is considered as the only strategy for the prevention of ZIKV-induced clinical issues. This study developed a plant-based recombinant vaccine that transiently expressed the ZIKV envelope protein (ZikaEnv:aghFc) in Nicotiana benthamiana and evaluated the protective immunity afforded by it in immunocompetent mice. ZikaEnv:aghFc induced both humoral and cellular immunity at a low dose (1-5 μg). This immune-inducing potential was enhanced further when adjuvanted CIA09A. In addition, antigen-specific antibodies and neutralizing antibodies were vertically transferred from immunized females to their progeny and afforded both protective immunity to ZIKV and cross-protection to Dengue virus infection. These results suggest that our plant-based ZIKV vaccine provides a safe and efficient protective strategy with a competitive edge.