Enhancing protein stability under stress: osmolyte-based deep eutectic solvents as a biocompatible and robust stabilizing medium for lysozyme under heat and cold shock
Anja Damjanović, Marijan Logarušić, Lidija‐Marija Tumir, Thanos Andreou, Marina Cvjetko Bubalo, Ivana Radojčić Redovniković
Abstract
. Investigation of the individual and combined effects of the DES components on enzyme stability confirmed the synergistic behavior of the kosmotrope-urea mixtures and the cumulative effects in kosmotrope-glycerol mixtures. Additionally, we have shown that the interplay between the enzyme's active and stable (but inactive) states is highly influenced by the water content in DESs. Finally, toxicity assessments of osmolyte-based DESs using cell lines (Caco-2, HaCaT, and HeLa) revealed no risks to human health.
Topics & Concepts
OsmolyteLysozymeBiocompatible materialEutectic systemChemical engineeringHeat stabilityChemistryMaterials scienceBiochemistryComposite materialCrystallographyMicrostructureBiomedical engineeringMedicineEngineeringEnzyme Catalysis and ImmobilizationAntimicrobial agents and applicationsSurfactants and Colloidal Systems