An integrated microfluidic chip for nucleic acid extraction and continued cdPCR detection of pathogens
Yaru Huang, Zehang Gao, Cong Ma, Yimeng Sun, Yuhang Huang, Chunping Jia, Jianlong Zhao, Shilun Feng
Abstract
copies per μL. Further on, a simulated clinical sample was used to test the system, and the integrated chip was able to accurately detect SARS-CoV-2 virus particle samples doped with interference (saliva) with a detection limit of 10 copies per μL. This integrated system could provide a promising tool for point-of-care testing of pathogenic infections.
Topics & Concepts
Nucleic acidDetection limitMicrofluidicsChromatographyChipLysisExtraction (chemistry)Microfluidic chipSample preparationNucleic acid quantitationElutionLab-on-a-chipChemistryNanotechnologyMaterials scienceComputer scienceBiochemistryTelecommunicationsBiosensors and Analytical DetectionSARS-CoV-2 detection and testingInnovative Microfluidic and Catalytic Techniques Innovation