Whole-brain optical access in a small adult vertebrate with two- and three-photon microscopy
Najva Akbari, Rose L. Tatarsky, Kristine E. Kolkman, Joseph R. Fetcho, Andrew H. Bass, Chris Xu
Abstract
brain without any modifications to the animal other than mechanical stabilization. Three-photon microscopy provides higher signal-to-background ratio and optical sectioning of fluorescently labeled vasculature through the deepest part of the brain, the hypothalamus. Hence, we use multiphoton microscopy to penetrate the entire adult brain within the geometry of this genus' head structures and without the need for pigment removal.
Topics & Concepts
VertebrateMicroscopyTwo-photon excitation microscopyZebrafishBiologyAnatomyLight sheet fluorescence microscopyNeuroscienceHuman brainOpticsPhysicsFluorescenceScanning confocal electron microscopyGeneBiochemistryAdvanced Fluorescence Microscopy TechniquesZebrafish Biomedical Research ApplicationsRetinal Development and Disorders