Autophagy is a novel pathway for neurofilament protein degradation <i>in vivo</i>
Mala V. Rao, Sandipkumar Darji, Philip Stavrides, Chris N. Goulbourne, Asok Kumar, Dun‐Sheng Yang, Lang Yoo, James Peddy, Ju‐Hyun Lee, Aidong Yuan, Ralph A. Nixon
Abstract
after intracerebroventricular infusion of 3-MA. Furthermore, using tomographic confocal microscopy, immunoelectron microscopy, and biochemical fractionation, we demonstrate the presence of NF proteins intra-lumenally within autophagosomes (APs), autolysosomes (ALs), and lysosomes (LYs). Our findings establish a prominent role for autophagy in NF proteolysis. Autophagy may regulate axon cytoskeleton size and responses of the NF cytoskeleton to injury and disease.
Topics & Concepts
AutophagyBiologyCell biologyBAG3ATG5LactacystinCalpainSequestosome 1Autophagy-related protein 13Protein subunitCytoskeletonNeurofilamentProteasomeKinaseProtein kinase ACellBiochemistryApoptosisProteasome inhibitorImmunologyEnzymeGeneProtein phosphorylationImmunohistochemistryAutophagy in Disease and TherapyParkinson's Disease Mechanisms and TreatmentsCellular transport and secretion