CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
Caroline Hoppe, Hilary L. Ashe
Abstract
CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila. For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).
Topics & Concepts
CRISPRGenome editingBiologyCas9GenomeDrosophila (subgenus)GeneComputational biologyGeneticsInsert (composites)CRISPR interferenceEngineeringMechanical engineeringCRISPR and Genetic EngineeringGenetics, Aging, and Longevity in Model OrganismsInsect symbiosis and bacterial influences