Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
Kanuj Mishra, Mariia Stankevych, J. Werner, Simon Grassmann, Vipul Gujrati, Yuanhui Huang, Uwe Klemm, Veit R. Buchholz, Vasilis Ntziachristos, André C. Stiel
Abstract
We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.
Topics & Concepts
In vivoPreclinical imagingMultiplexingTransgeneBiomedical engineeringChemistryBiologyComputer scienceMedicineBiochemistryGeneBiotechnologyTelecommunicationsPhotoacoustic and Ultrasonic ImagingNanoplatforms for cancer theranosticsAnalytical Chemistry and Sensors