NADPH oxidase overexpression and mitochondrial OxPhos impairment are more profound in human hearts donated after circulatory death than brain death
Nandan K. Mondal, Shiyi Li, A. Elsenousi, Aladdein Mattar, Katherine V. Nordick, Harveen K. Lamba, Camila Hochman-Mendez, Todd K. Rosengart, Kenneth K. Liao
Abstract
First human DCD heart research studied cardiac stress and mitochondrial dysfunction concerning WIT and the efficacy of del Nido cardioplegia as an organ procurement solution and subsequent cold storage. Mild to moderate cardiac stress and mitochondrial dysfunction were noticed in DCD hearts with WIT 20 and 40 min and cold storage for 4 and 2 h, respectively. These changes can serve as biomarkers, allowing interventions to preserve mitochondria and extend WIT in DCD hearts.
Topics & Concepts
Oxidative stressNADPH oxidaseOxidative phosphorylationMitochondrionNicotinamide adenine dinucleotide phosphateInternal medicineBiologyEndocrinologyReactive oxygen speciesOxidase testBiochemistryMedicineEnzymeTransplantation: Methods and OutcomesOrgan Transplantation Techniques and OutcomesCardiac Ischemia and Reperfusion