Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production
Miao Tian, Junying Fu, Zhiyuan Wang, Changlin Miao, Pengmei Lv, He Dong, Zhibing Li, Tao Liu, Ming Li, Wen Luo
Topics & Concepts
Rhizomucor mieheiLipaseThermostabilityChemistrySaturated mutagenesisPichia pastorisMethanolBiodieselBiochemistryBiodiesel productionMutantTriacylglycerol lipaseEnzymeOrganic chemistryRecombinant DNACatalysisGeneEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionElectrochemical sensors and biosensors