Studies of immunological and microbiological efficiency of the therapy of curcumin and methionine in the developed capsules
A. A. Khisamova, О. А. Гизингер, Н. В. Корнова, K. S. Zyryanova, А. M. Korkmazov, А. S. Beloshangin
Abstract
Curcumin is a polyphenolic compound, the main component of the Curcuma longa rhizome. Recently, there is growing interest to studies of this new, inexpensive and safe substance that may be used to treat various diseases. Curcumin is widely used in medicine due to its therapeutic efficacy and safety. Its usage in therapeutic practice as a dietary supplement has shown that curcumin exhibits antioxidant and immunomodulatory effects. Data from clinical studies have shown its pharmacokinetic, pharmacodynamic profile and potential use of curcumin in humans for treatment of various diseases, even at the early stages of treatment. Purpose of the study: analysis of immunomodulatory and microbiological properties when using the original grained preparation containing curcumin and methionine. It is suggested to have a regulatory effect by modulating microbial richness, diversity and composition of intestinal microflora. Analysis of immunomodulatory and microbiological activity of the components in the developed dosage form of the capsules with curcumin and methionine was carried out in vitro and in vivo. Functional and metabolic properties of neutrophils were determined with addition of its components, i.e., curcumin and methionine. The analysis of intensity and activity of neutrophils was carried out using the NBT-test. The analysis showed that simultaneous incubation of peripheral blood neutrophils from ICR (CD-1) mice with curcumin and methionine leads to an increase in spontaneous and induced NBT-reducing activity, an increase in the functional reserve and phagocytic activity of peripheral blood neutrophils in mice. A study of the modulating effects of oral intake of curcumin and methionine, which are part of the dosage form, on the intestinal microbiota of ICR (CD-1) mice was carried out. It was found that curcumin, together with methionine, affects the number of some representative families of intestinal microbial communities: in total, there were 640 common operating taxonomic units. between the curcumin-methionine and control groups, 65 were unique in the curcumin-methionine group and 93 in the control group. Given the direct link between gut microbiota and certain diseases, these results may help to interpret therapeutic benefits of curcumin with methionine. The results of the study showed that the developed dosage form, which contains curcumin and methionine, shows antioxidant and immunomodulatory effects, which can be potentially used to treat diseases associated with the effects of oxidative stress on the organism.