A novel systematic approach of co-steam processing: Enhancement of marker compounds in KIOM processed Polygonum multiflorum as ethnomedicine with improved ability of enzyme inhibition

Abstract

Polygonum multiflorum has been traditionally processed with black bean juice to reduce toxicity and enhance efficacy. The efficacy of KIOM processed P. multiflorum in osteoporosis treatment has been validated in previous research. The metabolites change in KIOM processed P. multiflorum by steaming with black bean juice has not been elucidated. In this study, metabolite differences between dried KIOM P. multiflorum and KIOM processed P. multiflorum were statistically verified. The efficacy improvement based on metabolite changes was identified through tyrosinase and elastase inhibition experiments. The analysis revealed significant differences in compound content between KIOM P-PM and KIOM D-PM, with higher levels of THSG, emodin, and physcion as efficacious compounds. In contrast, 5-hydroxymethylfurfural as toxic substance was decreased. KIOM P-PM exhibited strong inhibitory effects against tyrosinase and elastase, due to increase of THSG and emodin concentrations. This is the first report on systematic evaluation for metabolites change in P. multiflorum root tubers subjected to co-steam processing with additive and their chemical-specified efficacy. The KIOM P-PM, which has increased major compounds and efficacy through a co-steaming process from KIOM D-PM, is expected to have higher economic value compared to the general commercial P. multiflorum as industrial/in vitro cultivated medicinal products. • The main components of P. multiflorum were increased and toxic chemicals were decreased by co-steaming with black bean juice extract. • In the enzyme inhibition analysis base on the increased compounds, enzyme activity inhibition effect was increased. • A metabolite-based efficacy evaluation system for co-steaming process has been established as first scientific attempt, and verified as statistically.

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