A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution
Che Fai Alex Wong, Liisa van Vliet, Swapnil V. Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjørn T. Stokke, Florian Hollfelder, Rahmi Lale
Abstract
, with cell lysis triggered by inducer concentrations in the μM range. The LoD system yields sufficient release of the intracellularly produced enzymes to bring about a detectable quantity of product (measured by fluorescence in flow cytometry of double emulsions), while leaving viable cells for the downstream recovery of the genetic material.
Topics & Concepts
LysisEscherichia coliDirected evolutionMicrofluidicsMetagenomicsSingle-cell analysisCellFlow cytometryInducerHigh-throughput screeningWork flowBiologyChemistryBioprocessBiophysicsBiochemistryNanotechnologyMolecular biologyMaterials scienceMutantGenePaleontologyEngineeringIndustrial engineeringInnovative Microfluidic and Catalytic Techniques InnovationElectrowetting and Microfluidic TechnologiesMicrofluidic and Capillary Electrophoresis Applications