In Matrix Derivatization Combined with LC-MS/MS Results in Ultrasensitive Quantification of Plasma Free Metanephrines and Catecholamines
Martijn van Faassen, Rainer Bischoff, Karin Eijkelenkamp, Wilhelmina H. A. de Jong, Claude P. van der Ley, Ido P. Kema
Abstract
= 115 healthy individuals were established. Our work shows that analytes in the subnanomolar range in plasma can be derivatized in situ without any preceding sample extraction. The developed method shows improved sensitivity and selectivity over existing methods and enables simultaneous quantification of several classes of amines.
Topics & Concepts
ChemistryMetanephrinesDerivatizationChromatographyAnalyteMetanephrineLiquid chromatography–mass spectrometryMass spectrometryNormetanephrineHigh-performance liquid chromatographyUrineBiochemistryAdrenal and Paraganglionic TumorsCancer, Hypoxia, and MetabolismNeuroblastoma Research and Treatments