Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12
Xian‐Zheng Zhang, Ziwei Zhang, Yuchen Zhao, Lin Jin, Yu Tai, Yujing Tang, Shuo Geng, Han Zhang, Yufang Zhai, Yining Yang, Pin Pan, Peng He, Shuqi Fang, Chenlong Sun, Yu Chen, Mengqi Zhou, Lianghu Liu, Han Wang, Li Xu, Tianjing Zhang, Jinghan Hua, Hua Wang, Lingling Zhang
Abstract
experiments, pretreatment with NaCl enhances LPS-induced pyroptosis in RAW.264.7 and THP-1 cells through the p38 MAPK/NF-κB signaling pathway. Subsequent experiments reveal that Slc6a12 inhibitors and SGK1 silencing inhibit sodium-induced activation of macrophage pyroptosis and the p38 MAPK/NF-κB signaling pathway, whereas overexpression of the SGK1 gene counteracts the effect of sodium on macrophages. In conclusion, our findings verified that high salt intake promotes the progression of RA and provided a detailed elucidation of the activation of macrophage pyroptosis induced by sodium transportation through the Slc6a12 channel.