Chemical synthesis and immunological evaluation of entirely carbohydrate conjugate Globo H-PS A1
Samir Ghosh, Kevin R. Trabbic, Meng‐Chao Shi, Sharmeen Nishat, Pradheep Eradi, Kristopher A. Kleski, Peter R. Andreana
Abstract
an oxime linkage. Globo H-PS A1, formulated with adjuvants monophosphoryl lipid A and TiterMax® Gold. After immunization an antigen specific immune response was observed in ELISA with anti-Globo H IgG/IgM antibodies. Specificity of the corresponding antibodies was determined by FACS showing cell surface binding to Globo H-positive cancer cell lines MCF-7 and OVCAR-5. The anti-Globo H antibodies also exhibited complement-dependent cellular cytotoxicity against MCF-7 and OVCAR-5 cells.
Topics & Concepts
ConjugateCarbohydrateChemistryPolysaccharideBiochemistryCombinatorial chemistryStereochemistryMathematicsMathematical analysisGlycosylation and Glycoproteins ResearchCarbohydrate Chemistry and SynthesisCancer therapeutics and mechanisms