Efficacy of cleaning and sanitizing procedures to reduce Listeria monocytogenes on food contact surfaces commonly found in fresh produce operations
Erik Ohman, Samantha Kilgore, Joy Waite‐Cusic, Jovana Kovačević
Abstract
Cleaning and/or sanitizing methods were evaluated to reduce Listeria monocytogenes (Lm) on coupons of porous (PS; polyester-nylon with coating conveyor belt [PNCB], plywood [PW]) and non-porous (NPS; high density polyethylene, stainless steel) surfaces. Coupons (2.5 cm diameter) were inoculated with six-strain cocktail with cabbage juice, inverted and incubated on tryptic soy agar with yeast extract (TSAYE; 37 °C, 24 h). Coupons (n = 4; ∼9 log CFU/coupon) were rinsed only (RO), multi-step cleaned (MSC), sanitized only (SO; peroxyacetic acid [PAA], bleach, quaternary ammonium compounds [QAC]), or cleaned and sanitized (MSC + S), transferred to Dey/Engley broth (DEB) with glass beads (1 g), vortexed and enumerated on Harlequin-TSAYE. Half of the coupons were dried prior to transfer to DEB. MSC resulted in ave. 2 log CFU/coupon reductions on NPS, and 0.6-1.1 log on PS. MSC + S led to >5-log reduction on NPS (81%; n = 48). On PS, MSC + S-PAA resulted in 1.8 and 1.9 log reductions on PW and PNCB, respectively. RO and SO reduced Lm on all surfaces, although less effectively than MSC and MSC + S. On PS, MSC + S-PAA was most effective, followed by bleach and QAC (p < 0.05). Drying after sanitizing increased Lm reduction by ∼0.4 log (p < 0.05). Partial cleaning and/or sanitizing leads to minimal reduction of Lm, while multi-step cleaning with sanitizing is highly effective.