Proximity-Labeling Reveals Novel Host and Parasite Proteins at the <i>Toxoplasma</i> Parasitophorous Vacuole Membrane
Alicja M. Cygan, Pierre M. Jean Beltran, Alma G. Mendoza, Tess C. Branon, Alice Y. Ting, Steven A. Carr, John C. Boothroyd
Abstract
is an intracellular pathogen which resides and replicates inside a membrane-bound vacuole in infected cells. This vacuole is modified by both parasite and host proteins which participate in a variety of host-parasite interactions at this interface, including nutrient exchange, effector transport, and immune modulation. Only a small number of parasite and host proteins present at the vacuolar membrane and exposed to the host cytosol have thus far been identified. Here, we report the identification of several novel parasite and host proteins present at the vacuolar membrane using enzyme-catalyzed proximity-labeling, significantly increasing our knowledge of the molecular players present and novel biology occurring at this crucial interface.