Quantitative Characterization of Three Carbonic Anhydrase Inhibitors by LESA Mass Spectrometry
Eva Illes‐Toth, Christopher J. Stubbs, Emma K. Sisley, Jeddidiah Bellamy‐Carter, Anna L. Simmonds, Todd H. Mize, Iain B. Styles, Richard J. A. Goodwin, Helen J. Cooper
Abstract
values determined from LESA MS measurements where the ligand was in the sampling solvent were inconsistent. Our results suggest that LESA MS is a suitable tool for quantitative analysis of protein-ligand interactions when the dried sample comprises both protein and ligand.
Topics & Concepts
ChemistryMass spectrometryLigand (biochemistry)Dissociation constantCarbonic anhydraseAnalytical Chemistry (journal)ChromatographySubstrate (aquarium)BiochemistryEnzymeReceptorGeologyOceanographyMass Spectrometry Techniques and ApplicationsEnzyme function and inhibitionAnalytical chemistry methods development