Chiral voltammetric sensor on the basis of nanosized MFI zeolite for recognition and determination of tryptophan enantiomers
R. A. Zilberg, Yu. B. Teres, Marat Agliulin, Ivan Vakulin, Elena O. Bulysheva, М. В. Сычева, В. Н. Майстренко
Abstract
Abstract A voltammetric sensor comprising a paste electrode made of graphitized thermal carbon black CarboblackC and nanosized zeolite MFI was developed for selective recognition and determination of tryptophan enantiomers. It was found that in determining tryptophan enantiomers, the dependence remains linear at concentrations from 5×10 −5 to 1×10 −3 M. The detection limits of tryptophan L‐ and D‐enantiomers were calculated to be 4.98×10 −7 M and 3.20×10 −7 M, while their lower limits of determinable concentrations–1.66×10 −6 M and 1.07×10 −6 M, respectively. The sensor is more sensitive to D‐tryptophan (Δ E p =20 mV, i pD / i pL =1.58). The developed sensor was shown to successfully determine tryptophan enantiomers in model solutions of pure enantiomers, in a mixture of enantiomers, in biological fluids, and in tablet forms of biologically active additives. It was shown that the suggested sensor made it possible to determine the ratio of L‐ and D‐enantiomers of tryptophan in a mixture with a high probability.