Murine Intrarectal Instillation of Purified Recombinant <i>Clostridioides difficile</i> Toxins Enables Mechanistic Studies of Pathogenesis
Nicholas O. Markham, Sarah C. Bloch, John A. Shupe, Erin N. Laubacher, Audrey K. Thomas, Heather K. Kroh, Kevin O. Childress, F. Christopher Peritore‐Galve, M. Kay Washington, Robert J. Coffey, D. Borden Lacy
Abstract
Clostridioides difficile is linked to nearly 225,000 antibiotic-associated diarrheal infections and almost 13,000 deaths per year in the United States. Pathogenic strains of C. difficile produce toxin A (TcdA) and toxin B (TcdB), which can directly kill cells and induce an inflammatory response in the colonic mucosa.
Topics & Concepts
ClostridioidesMicrobiologyClostridium difficile toxin BToxinBiologyPathogenesisClostridium difficile toxin ADiarrheal diseasesRecombinant DNAEnterotoxinMicrobial toxinsDiarrheaAntibioticsClostridium difficileVirologyImmunologyMedicinePathologyEscherichia coliGeneBiochemistryClostridium difficile and Clostridium perfringens researchMicroscopic ColitisGastrointestinal motility and disorders