Single-cell ionic current phenotyping explains stem cell-derived cardiomyocyte action potential morphology
Alexander P. Clark, Siyu Wei, Kristin E Fullerton, Trine Krogh‐Madsen, David J. Christini
Abstract
We present rapid ionic current phenotyping (RICP), a current quantification approach based on an optimized voltage-clamp protocol. The method captures a rich snapshot of the ionic current dynamics, providing quantitative information about multiple currents (e.g., I Ca,L , I Kr ) in the same cell. The protocol helped to identify key ionic determinants of cellular action potential heterogeneity in iPSC-CMs. This included unexpected results, such as the critical role of I Kr in establishing the maximum diastolic potential.
Topics & Concepts
Patch clampElectrophysiologyInduced pluripotent stem cellBiophysicsCurrent clampInward-rectifier potassium ion channelMembrane potentialChemistryNeuroscienceIon channelBiologyBiochemistryGeneEmbryonic stem cellReceptorCardiac electrophysiology and arrhythmiasNeuroscience and Neural EngineeringIntegrated Circuits and Semiconductor Failure Analysis