Metabolic Rearrangements Causing Elevated Proline and Polyhydroxybutyrate Accumulation During the Osmotic Adaptation Response of Bacillus megaterium
Thibault Godard, Daniela Zühlke, Georg Richter, Melanie W Wall, Manfred Rohde, Katharina Riedel, Ignacio Poblete‐Castro, Rainer Krull, Rebekka Biedendieck
Abstract
redirected the produced acetyl-CoA toward the polyhydroxybutyrate (PHB) biosynthetic pathway accumulating around 30% of the cell dry weight (CDW) as PHB. This direct relation between osmotic stress and intracellular PHB content has been evidenced for the first time, thus opening new avenues for synthesizing this valuable biopolymer using varying salt concentrations under non-limiting nutrient conditions.
Topics & Concepts
Bacillus megateriumBiochemistryOsmotic shockPentose phosphate pathwayPolyhydroxybutyrateCitric acid cycleOsmoprotectantNAD+ kinaseProlineBiologyMetabolic pathwayOsmotic pressureGlycolysisChemistryEnzymeAmino acidBacteriaGeneGeneticsbiodegradable polymer synthesis and propertiesBiopolymer Synthesis and ApplicationsPolyamine Metabolism and Applications