Probing the structure of nanodiscs using surface-induced dissociation mass spectrometry
Sophie R. Harvey, Zachary L. VanAernum, Marius M. Kostelic, Michael T. Marty, Vicki H. Wysocki
Abstract
In the study of membrane proteins and antimicrobial peptides, nanodiscs have emerged as a valuable membrane mimetic to solubilze these molecules in a lipid bilayer. We present the structural characterization of nanodiscs using native mass spectrometry and surface-induced dissociation, which are powerful tools in structural biology.
Topics & Concepts
Dissociation (chemistry)Mass spectrometryChemistryCollision-induced dissociationInfrared multiphoton dissociationTandem mass spectrometryChromatographyPhysical chemistryMass Spectrometry Techniques and ApplicationsIon-surface interactions and analysisAnalytical chemistry methods development