Combination of ultrashort PCR and <i>Pyrococcus furiosus</i> Argonaute for DNA detection
Ruyi He, Longyu Wang, Fei Wang, Jun Yang, Xiao Yu, Yuan Wang, Zhiguo Liu, Chunhua Li, Lixin Ma
Abstract
We used small primers to obtain guides from the target DNA to mediate Pf Ago cutting of molecular beacons, a fast and effective method.
Topics & Concepts
Pyrococcus furiosusMolecular beaconArgonauteNucleic acidDNACleavage (geology)ChemistryComputational biologyMolecular biologyBiologyGeneBiochemistryRNAOligonucleotideRNA interferenceArchaeaPaleontologyFracture (geology)CRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms