Fluorescence and colorimetric dual-mode multienzyme cascade nanoplatform based on CuNCs/FeMn-ZIF-8/PCN for detection of sarcosine
Yu Wu, Chenxi Ke, Zichen Song, Hongda Zhu, Huiling Guo, Hongmei Sun, Mingxing Liu
Abstract
generated in the cascade caused the FL quenching of nanocomposites and the discoloration of 3,3',5,5'-tetramethylbenzidin. The linear ranges for SA detection in the dual-mode system were 1-100 μM (FL) and 1-200 μM (colorimetric), with detection limits of 0.34 and 0.59 μM, respectively. This nanoplatform exhibited notable repeatability, specificity, and stability, making it suitable for detecting sarcosine in real human urine samples. Therefore, this dual-mode multienzyme cascade nanoplatform would have a potential applicative prospect for detecting SA and other biomarkers in real clinical samples.
Topics & Concepts
SarcosineChemistryDual modeFluorescenceDetection limitCascadeChromatographyNanotechnologyMaterials scienceBiochemistryPhysicsQuantum mechanicsAerospace engineeringEngineeringGlycineAmino acidAdvanced Nanomaterials in CatalysisCarbon and Quantum Dots ApplicationsNanocluster Synthesis and Applications