Correlation of SARS-CoV-2 Nucleocapsid Antigen and RNA Concentrations in Nasopharyngeal Samples from Children and Adults Using an Ultrasensitive and Quantitative Antigen Assay
Nira R. Pollock, Timothy J. Savage, Hanna Wardell, Rose Lee, Anu Mathew, Martin Stengelin, George B. Sigal
Abstract
Diagnosis of COVID-19 by PCR offers high sensitivity, but the utility of detecting samples with high cycle threshold ( C T ) values remains controversial. Currently available rapid diagnostic tests (RDTs) for SARS-CoV-2 nucleocapsid antigens (Ag) have sensitivity well below PCR.
Topics & Concepts
ImmunoassayAntigenConfidence intervalDetection limitMedicineSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyMolecular biologyBiologyCoronavirus disease 2019 (COVID-19)ChemistryChromatographyInternal medicineImmunologyAntibodyInfectious disease (medical specialty)DiseaseSARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchAdvanced biosensing and bioanalysis techniques