Structural Basis of Pore Formation in the Mannose Phosphotransferase System by Pediocin PA-1
Liyan Zhu, Jianwei Zeng, Chang Wang, Jiawei Wang
Abstract
species. The mannose phosphotransferase system (man-PTS) is responsible for the sensitivity of Listeria monocytogenes to pediocin PA-1. In this study, we report the cryo-EM structures of man-PTS from Listeria monocytogenes alone and its complex with pediocin PA-1 at resolutions of 3.12 and 2.45 Å, respectively. Our results facilitate the understanding of the mode of action of class IIa bacteriocins as an alternative to antibiotics.
Topics & Concepts
BacteriocinListeria monocytogenesPEP group translocationListeriaMannoseBiochemistryPhosphotransferaseBiologyMicrobiologyBacteriaAntimicrobial peptidesExtracellularAntimicrobialChemistryEscherichia coliAntibacterial activityPathogenic bacteriaCell membraneCytoplasmMembraneAcetobacterBacterial cell structureAntibacterial agentPseudomonas aeruginosaListeria monocytogenes in Food SafetyEnterobacteriaceae and Cronobacter ResearchFungal and yeast genetics research