Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts <i>In Vivo</i> Outcomes in Galleria mellonella
Sarah Miller, Kristine Goy, Rosemary C. She, Brad Spellberg, Brian Luna
Abstract
Antimicrobial susceptibility testing (AST) in RPMI 1640, a more physiologically relevant culture medium, revealed that a substantial proportion of carbapenem-resistant Acinetobacter baumannii isolates were susceptible to azithromycin, a macrolide antibiotic not currently considered effective against A. baumannii . Experiments using Galleria mellonella validated these in vitro data.
Topics & Concepts
Galleria mellonellaAcinetobacter baumanniiMicrobiologyAzithromycinAntimicrobialIn vivoCarbapenemCephalosporinBiologyAcinetobacterMedicineAntibioticsBacteriaPseudomonas aeruginosaVirulenceBiotechnologyBiochemistryGeneGeneticsInvertebrate Immune Response MechanismsAquaculture disease management and microbiotaMalaria Research and Control