Alteration of the abundance of <em>Parvimonas&nbsp;micra</em> in the gut along the adenoma‑carcinoma sequence
Jun Xu, Min Yang, Dongyan Wang, Shuilong Zhang, Su Yan, Yongliang Zhu, Weichang Chen
Abstract
<em>Parvimonas micra</em> (<em>P. micra</em>) is reported to be associated with colorectal cancer (CRC). However, its association with colorectal adenoma (CRA) and its role in the initiation of colorectal tumors remain unknown. The present study aimed to clarify the relationship between <em>P. micra</em> and CRA and CRC by exploring the changes of <em>P. micra</em> abundance in an adenoma‑carcinoma sequence in a new cohort and 4 public sequencing datasets. To investigate the alterations of <em>P. micra</em> abundance in the gut along the adenoma‑carcinoma sequence, quantitative PCR (qPCR) was conducted to measure the relative abundance of <em>P. micra</em> in fecal samples from 277 subjects (128 patients with CRA, 66 patients with CRC and 83 healthy individuals, as controls) who underwent colonoscopy as outpatients. Then, the relative abundance of <em>P. micra</em> was analyzed in fecal samples from 596 subjects (185 healthy controls, 158 CRC, 253 CRA) in four public 16S rRNA sequencing datasets. The qPCR results demonstrated that the CRA group had an abundance of <em>P. micra</em> (P=0.2) similar to that of the healthy control group, while the CRC group had a significantly increased abundance (P=8.2x10<sup>‑11</sup>). The level of <em>P. micra</em> effectively discriminated patients with CRC from healthy controls, while it poorly discriminated patients with CRA from healthy controls; with an area under the receiver operating characteristic curve of 0.867 for patients with CRC and 0.554 for patients with CRA. The same pattern of the alteration of <em>P. micra</em> abundance, which was low in healthy controls and patients with CRA but elevated in patients with CRC, was found in all four public sequencing datasets. These results suggested that <em>P. micra</em> was closely associated with, and may serve as a diagnostic marker for, CRC but not CRA. Moreover, it was indicated that <em>P. micra</em> may be an opportunistic pathogen of CRC, which may promote CRC development but serve a limited role in tumorigenesis.