Engineered protein cages for selective heparin encapsulation
Salla Välimäki, Qing Liu, Lise Schoonen, Daan F. M. Vervoort, Nonappa Nonappa, Veikko Linko, Roeland J. M. Nolte, Jan C. M. van Hest, Mauri A. Kostiainen
Abstract
A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.
Topics & Concepts
Encapsulation (networking)Materials scienceHeparinNanotechnologyChemistryBiochemistryComputer scienceComputer securityProteoglycans and glycosaminoglycans researchGlycosylation and Glycoproteins ResearchMonoclonal and Polyclonal Antibodies Research