A hydrogel with supramolecular surface functionalization for cancer cell capture and multicellular spheroid growth and release
Yuting Wen, Nana Nyarko Mensah, Xia Song, Jingling Zhu, Wui Siew Tan, Xinwei Chen, Jun Li
Abstract
A hydrogel scaffold with a non-fouling but specific cancer cell-adhesive surface was fabricated through surface modification using β-cyclodextrin-based host-guest chemistry. Interestingly, the hydrogel surface not only selectively captured specific cancer cells, but also grew the cells into multicellular spheroids. The spheroids could be released without damaging the cell viability through replacing the host moieties on the scaffold, and the released spheroids showed no changes in size or morphology.
Topics & Concepts
SpheroidSurface modificationCancer cellScaffoldMulticellular organismChemistrySupramolecular chemistryBiophysicsViability assayCellMorphology (biology)NanotechnologyMaterials scienceCancerIn vitroBiomedical engineeringBiochemistryBiologyMoleculeOrganic chemistryPhysical chemistryGeneticsMedicine3D Printing in Biomedical ResearchHydrogels: synthesis, properties, applicationsCellular Mechanics and Interactions