Local delivery of mRNA-encoded cytokines promotes antitumor immunity and tumor eradication across multiple preclinical tumor models
C. Hotz, Timothy R. Wagenaar, Friederike Gieseke, Dinesh S. Bangari, Michelle Callahan, Hui Cao, Jan Diekmann, Mustafa Diken, Christian Grunwitz, Andy Hebert, Karl Hsu, Marie Bernardo, Katalin Karikó, Sebastian Kreiter, Andreas N. Kuhn, Mikhail Levit, Natalia V. Malkova, Serena Masciari, Jack Pollard, Hui Qü, Sue Ryan, Abderaouf Selmi, Julia Schlereth, Kuldeep Singh, Fangxian Sun, Bodo Tillmann, Tatiana Tolstykh, William Weber, Lena Wicke, Sonja Witzel, Qunyan Yu, Yuan Zhang, Gang Zheng, Joanne Lager, Gary J. Nabel, Uğur Şahin, Dmitri Wiederschain
Abstract
T cell infiltration, and formation of immune memory. Antitumor activity extended beyond the treated lesions and inhibited growth of distant tumors and disseminated tumors. Combining the mRNAs with immunomodulatory antibodies enhanced antitumor responses in both injected and uninjected tumors, thus improving survival and tumor regression. Consequently, clinical testing of this cytokine-encoding mRNA mixture is now underway.