Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
Chasity P. Janosko, Olivia Shade, Taylor M. Courtney, Trevor J. Horst, Melinda Liu, Sagar D. Khare, Alexander Deiters
Abstract
High Resolution Image Download MS PowerPoint Slide An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans -configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.
Topics & Concepts
Genetic codePhenylalanineTranslation (biology)Amino acidGeneChemistrySelectivityGeneticsProtein biosynthesisBiologyBiochemistryMessenger RNACatalysisPhotochromic and Fluorescence ChemistryPhotoreceptor and optogenetics researchbioluminescence and chemiluminescence research