Spirometra mansoni sparganosis identified by metagenomic next-generation sequencing: a case report
Diefei Hu, Wenxiang Jin, Huarong Ding, Yu Pang, Shasha Ma, Min Yang, Shuwen Wu, Muliang Jiang, Lingling Pang, Shuming Luo, Qiuyue Ning
Abstract
A 30-year-old male patient had a cyst on the left hip and progressive enlargement for more than 2 months. Combined blood tests, magnetic resonance imaging, and pathology findings, cysticercosis infection was suspected. However, the treatment for cysticercosis was ineffective. We conducted a metagenomic next-generation sequencing (mNGS) analysis on the formalin-fixed, paraffin-embedded specimen of the patient's surgically excised tissue, and the results suggested Spirometra mansoni, mNGS was further confirmed by polymerase chain reaction and phylogenetic analysis of cytochrome c oxidase subunit 1 (cox1) gene. Based on these results, we found that mNGS provided a better method of diagnosing parasitic infections.