A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a
Rongxing Zhou, Yongya Li, Tianyu Dong, Yanan Tang, Feng Li
Abstract
Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.
Topics & Concepts
Loop-mediated isothermal amplificationCRISPRSequence (biology)Loop (graph theory)PlasmonComputational biologyChemistryMolecular biologyPhysicsBiologyGeneticsOptoelectronicsDNAGeneMathematicsCombinatoricsBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic Engineering